Recombinant Human CENPE Protein

• Cat.No.: CENPE-021H
• Product Overview: CENPE Protein, Human is the recombinant human-derived CENPE, expressed by E. coli

Specification

• Species: Human
• Source: E.coli
• Protein Length: 1-339 aa
• Description: Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.
• Form: Solution. Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
• Molecular Mass: 38.2 kDa
• Endotoxin: <1 EU/μg, determined by LAL method.
• Purity: Greater than 90% as determined by reducing SDS-PAGE
• Storage: Stored at -80 centigrade for 1 year from date of receipt. It is stable at -20 centigrade for 3 months after opening. It is recommended to freeze aliquots at -80 centigrade for extended storage. Avoid repeated freeze-thaw cycles.
• Shipping: Shipping with dry ice.
• Reconstitution: Please use rapid thawing with running water to thaw the protein.

Gene Information

• Gene Name: CENPE centromere protein E [ Homo sapiens (human) ]
• Official Symbol: CENPE
• Synonyms: CENPE; centromere protein E; KIF10; CENP-E; MCPH13; PPP1R61; centromere-associated protein E; Centromere autoantigen E (312kD); centromere protein E, 312kDa; kinesin family member 10; kinesin-7; kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61
• Gene ID: 1062
• mRNA Refseq: NM_001813
• Protein Refseq: NP_001804
• MIM: 117143
• UniProt ID: Q02224