Recombinant Human CENPH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human CENPH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : CENPH-745H
Product Overview : CENPH MS Standard C13 and N15-labeled recombinant protein (NP_075060) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 28.5 kDa
AA Sequence : MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT KQQLLEYKSM VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK LALDRMRLST ALKKNLEKIS RQSSVLMDNM KHLLELNKLI MKSQQESWDL EEKLLDIRKK RLQLKQASES KLLEIQTEKN KQKIDLDSME NSERIKIIRQ NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL EKNVDMMTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : CENPH centromere protein H [ Homo sapiens (human) ]
Official Symbol : CENPH
Synonyms : CENPH; centromere protein H; NNF1; MIND kinetochore complex component; homolog (S. cerevisiae); PMF1; CENP-H; kinetochore protein CENP-H; interphase centromere complex protein 35;
Gene ID : 64946
mRNA Refseq : NM_022909
Protein Refseq : NP_075060
MIM : 605607
UniProt ID : Q9H3R5

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