Recombinant Human CES1 Protein


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Recombinant Human CES1 Protein

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Cat.No. : CES1-106H
Product Overview : Recombinant human CES1 protein without tag was expressed in E. coli.
Description : This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
Source : E. coli
Species : Human
Bio-activity : CES Activity Data(Unless otherwise stated, all assays are carried out at 30 centigrade in 50 mM potassium phosphate buffer (pH 7.4)/acetonitrile, 98/2 (v/v))
Substrate: 4-nitrophenyl butyrate
Linear with time up to: 10 min(Determined at 90 μM substrate, using a protein concentration of 15 μg/ml)
Linear with protein up to: 50 μg/ml(Determined at 90 μM substrate)
Vmax: 511 nmol/min/mg protein
Km: 152 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : Protein Concentration: 10 mg/ml
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CES1 carboxylesterase 1 [ Homo sapiens (human) ]
Official Symbol : CES1
Synonyms : CES1; carboxylesterase 1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1; liver carboxylesterase 1; acyl coenzyme A:cholesterol acyltransferase; brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); carboxylesterase 2 (liver); cholesteryl ester hydrolase; cocaine carboxylesterase; egasyn; human monocyte/macrophage serine esterase 1; methylumbelliferyl-acetate deacetylase 1; monocyte/macrophage serine esterase; retinyl ester hydrolase; serine esterase 1; triacylglycerol hydrolase
Gene ID : 1066
mRNA Refseq : NM_001025194
Protein Refseq : NP_001020365
MIM : 114835
UniProt ID : P23141

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