"CHEK1" Related Products

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Recombinant Human CHEK1 Protein, GST-tagged

Cat.No.: CHEK1-229H
Product Overview: Recombinant human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.
Source: Baculovirus-Insect Cells
Species: Human
Tag: GST
Predicted N Terminal: Met
Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH.
Bio-activity: The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
Molecular Mass: The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: CHEK1 checkpoint kinase 1 [ Homo sapiens ]
Official Symbol: CHEK1
Synonyms: CHK1; CHK1 checkpoint homolog; Checkpoint, S. pombe, homolog of, 1; Chk1-S; cell cycle checkpoint kinase; checkpoint kinase-1
Gene ID: 1111
mRNA Refseq: NM_001114121
Protein Refseq: NP_001107593
MIM: 603078
UniProt ID: O14757
Chromosome Location: 11q24.2
Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell cycle, conserved biosystem; DNA Double-Strand Break Repair, organism-specific biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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