| Cat.No. : |
CCL3-79H
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Recombinant Human chemokine (C-C motif) ligand 3 G0S19-1 protein sequence (containing the signal peptide sequence, and the mature human Interleukin 4 sequence) was expressed in modifiedhuman 293 cells. |
| Description : |
Human Macrophage inflammatory protein-1alpha (MIP-1 alpha; MIP-1a) belongs to the chemokine family. Chemokines are small secreted molecules containing 4 conserved cysteine residues and 2 disulfide linkages. MIP-1 alpha is constitutively expressed from platelets and bone marrow CD34 progenitor cells. MIP-1 alpha expression can also be induced by bacteria or bacterial products such as LPS in monocyte/macrophages, dendritic cells, neutrophils, microglial cells and in activated B and T cells. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
ADTPTACCFSYT SRQIPQNFIAD YFETS SQCS KPGVIFL TKRSRQV CADPSE EW VQ KY VSDLELSA. |
| Molecular Mass : |
MIP-1 alpha migrates in SDS-PAGE at approximately 10 kDa. This compares with the predicted molecular mass of 7.4kDa. |
| PI : |
MIP-1 alpha migrates in 2D PAGE with an observed pI of 4.8. This compares with the predicted pI of 4.77. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Working concentrations of MIP-1 alpha should be less than 100ng/ml to avoid aggregation. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| PDB rendering based on 1b50. : |
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
