Recombinant Human CHMP4B Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human CHMP4B Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : CHMP4B-3377H
Product Overview : CHMP4B MS Standard C13 and N15-labeled recombinant protein (NP_789782) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 25 kDa
AA Sequence : MSVFGKLFGA GGGKAGKGGP TPQEAIQRLR DTEEMLSKKQ EFLEKKIEQE LTAAKKHGTK NKRAALQALK RKKRYEKQLA QIDGTLSTIE FQREALENAN TNTEVLKNMG YAAKAMKAAH DNMDIDKVDE LMQDIADQQE LAEEISTAIS KPVGFGEEFD EDELMAELEE LEQEELDKNL LEISGPETVP LPNVPSIALP SKPAKKKEEE DDDMKELENW AGSMTRTRPL EQKLISEEDL AANDILDYKD DDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : CHMP4B charged multivesicular body protein 4B [ Homo sapiens (human) ]
Official Symbol : CHMP4B
Synonyms : CHMP4B; charged multivesicular body protein 4B; C20orf178, chromatin modifying protein 4B, chromosome 20 open reading frame 178; charged multivesicular body protein 4b; dJ553F4.4; Shax1; SNF7 2; VPS32B; hSnf7-2; hVps32-2; chromatin modifying protein 4B; chromatin-modifying protein 4b; SNF7 homolog associated with Alix 1; Snf7 homologue associated with Alix 1; vacuolar protein sorting-associated protein 32-2; vacuolar protein-sorting-associated protein 32-2; SNF7; CTPP3; CHMP4A; SNF7-2; Vps32-2; C20orf178;
Gene ID : 128866
mRNA Refseq : NM_176812
Protein Refseq : NP_789782
MIM : 610897
UniProt ID : Q9H444

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