Recombinant Human Ciliary Neurotrophic Factor, His-tagged

• Cat.No.: CNTF-08H
• Product Overview: Recombinant human CNTF, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques(220 aa, 25 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT.
• Molecular Weight: 25 kDa (220 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CNTF ciliary neurotrophic factor [ Homo sapiens ]
• Synonyms: HCNTF; CNTF; Ciliary Neurotrophic Factor; OTTHUMP00000174731; ciliary neurotrophic factor.
• Gene ID: 1270
• mRNA Refseq: NM_000614
• Protein Refseq: NP_000605
• MIM: 118945
• UniProt ID: P26441
• Chromosome Location: 11q12.2
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway.
• Function: ciliary neurotrophic factor receptor binding; interleukin-6 receptor binding; protein binding.