Recombinant Human CLK2 Protein, GST-tagged

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Recombinant Human CLK2 Protein, GST-tagged

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Cat.No. : CLK2-1494H
Product Overview : Human CLK2 full-length ORF ( AAH14067, 1 a.a. - 498 a.a.) recombinant protein with GST-tag at N-terminal.
Description : This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 80.52 kDa
AA Sequence : MPHPRRYHSSERGSR GSYREHYRSRKHKRR RSRSWSSSSDRTRRR RREDSYHVRSRSSYD DRSSDRRVYDRRYCG SYRRNDYSRDRGDAY YDTDYRHSYEYQREN SSYRSQRSSRRKHRR RRRRSRTFSRSSSHS SRRAKSVEDDAEGHL IYHVGDWLQERYEIV STLGEGTFGRVVQCV DHRRGGARVALKIIK NVEKYKEAARLEINV LEKINEKDPDNKNLC VQMFDWFDYHGHMCI SFELLGLSTFDFLKD NNYLPYPIHQVRHMA FQLCQAVKFLHDNKL THTDLKPENILFVNS DYELTYNLEKKRDER SVKSTAVRVVDFGSA TFDHEHHSTIVSTRH YRAPEVILELGWSQP CDVWSIGCIIFEYYV GFTLFQTHDNREHLA MMERILGPIPSRMIR KTRKQKYFYRGRLDW DENTSAGRYVRENCK PLRRYLTSEAEEHHQ LFDLIESMLEYEPAK RLTLGEALQHPFFAR LRAEPPNKLWDSSRD ISR
Applications : Kinase Assay enlarge
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing: :

12.5% SDS-PAGE Stained with Coomassie Blue.

Kinase Assay :

Gene Name : CLK2 CDC-like kinase 2 [ Homo sapiens ]
Official Symbol : CLK2
Synonyms : CLK2; CDC-like kinase 2; dual specificity protein kinase CLK2; clk2; CLK kinase; hCLK2; MGC61500;
Gene ID : 1196
mRNA Refseq : NM_003993
Protein Refseq : NP_003984
MIM : 602989
UniProt ID : P49760

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