Recombinant Human CLOCK, GST-tagged

• Cat.No.: CLOCK-11340H
• Product Overview: Recombinant Human CLOCK protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-351a.a.
• Description: This gene product regulates circadian rhythm and metabolism. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and a DNA binding histone acetyltransferase. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.
• Publications:
  Diurnal oscillations of endogenous H2O2 sustained by p66Shc regulate circadian clocks (2019)

Gene Information

• Gene Name: CLOCK clock homolog (mouse) [ Homo sapiens ]
• Official Symbol: CLOCK
• Synonyms: CLOCK; clock homolog (mouse); clock (mouse) homolog; circadian locomoter output cycles protein kaput; bHLHe8; KAT13D; KIAA0334; class E basic helix-loop-helix protein 8; circadian locomoter output cycles kaput protein
• Gene ID: 9575
• mRNA Refseq: NM_004898
• Protein Refseq: NP_004889
• MIM: 601851
• UniProt ID: O15516
• Chromosome Location: 4q12
• Pathway: BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Circadian rhythm - mammal, organism-specific biosystem; Circadian rhythm - mammal, conserved biosystem; Circadian rhythm pathway, organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem
• Function: DNA binding; E-box binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; RNA polymerase II transcription factor