"COL10A1" Related Products

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Recombinant Human COL10A1 protein, His-tagged

Cat.No.: COL10A1-490H
Product Overview: Recombinant Human COL10A1(Thr547~Met680) fused with His tag at N-terminal was expressed in E. coli.
Description: This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).
Source: E. coli
Species: Human
Tag: His
Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass: 16.3kDa
Protein length: Thr547~Met680
AA Sequence: TGMPVSAFTVUKSK AYPAIGTPIP FDKILYNRQQ HYDPRTGIFT CQIPGIYYFS YHVHVKGTHV WVGLYKNGTP VMYTYDEYTK GYLDQASGSA IIDLTENDQV WLQLPNAESN GLYSSEYVHS SFSGFLVAPM
Endotoxin: <1.0EU per 1µg (determined by the LAL method)
Purity: > 90%
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigradefor 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigradefor one month. Aliquot and store at -80 centigradefor 12 months.
Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name: COL10A1 collagen, type X, alpha 1 [ Homo sapiens ]
Official Symbol: COL10A1
Synonyms: COL10A1; collagen, type X, alpha 1; collagen alpha-1(X) chain; Schmid metaphyseal chondrodysplasia; collagen X, alpha-1 polypeptide;
Gene ID: 1300
mRNA Refseq: NM_000493
Protein Refseq: NP_000484
MIM: 120110
UniProt ID: Q03692
Chromosome Location: 6q21-q22
Pathway: Endochondral Ossification, organism-specific biosystem; Senescence and Autophagy, organism-specific biosystem;
Function: metal ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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