Recombinant Human COL7A1 Cell Lysate, Myc/DDK-tagged

• Cat.No.: COL7A1-30HL
• Product Overview: Transient overexpression lysate of collagen, type VII, alpha 1 (COL7A1) with a C-Myc/DDK tag was expressed in HEK293T.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer (25 mM Tris-HCl pH7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1×Proteinase inhibitor cocktail mix, 1 mM PMSF and 1 mM Na3VO4)
  1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100 mM DTT)

Specification

• Description: This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 295.22 kDa
• Stability: Avoid repeated freeze-thaw cycles.
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Reconstitution: Lysate samples can be diluted with 2×SDS Sample Buffer provided.

Gene Information

• Gene Name: COL7A1 collagen type VII alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL7A1
• Synonyms: COL7A1; collagen type VII alpha 1 chain; EBD1; EBR1; EBDCT; NDNC8; collagen alpha-1(VII) chain; LC collagen; collagen VII, alpha-1 polypeptide; collagen, type VII, alpha 1; long-chain collagen
• Gene ID: 1294
• mRNA Refseq: NM_000094
• Protein Refseq: NP_000085
• MIM: 120120
• UniProt ID: Q02388