Recombinant Human CREBBP protein, His&FLAG-tagged

• Cat.No.: CREBBP-2815H
• Product Overview: Recombinant Human CREBBP protein(accession number NM_004380.2)(1081-1197 aa) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His
• Protein Length: 1081-1197 aa
• Form: Supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol.
• Bio-activity: 3 μM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of CREBBP (1081-1197) protein in 10 μl reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 μl FLAG antibody and SA-XL665 mixture (1:100 dilution in reaction buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
• Molecular Mass: 18.7 kDa
• Purity: >85% pure by SDS-PAGE.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CREBBP CREB binding protein [ Homo sapiens ]
• Official Symbol: CREBBP
• Synonyms: CREBBP; CREB binding protein; RSTS, Rubinstein Taybi syndrome; CREB-binding protein; CBP; KAT3A; RTS; RSTS
• Gene ID: 1387
• mRNA Refseq: NM_001079846
• Protein Refseq: NP_001073315
• MIM: 600140
• UniProt ID: Q92793