Active Recombinant Human CRLF2 protein, Fc-tagged

• Cat.No.: CRLF2-373H
• Product Overview: Recombinant Human CRLF2 protein(Gly 25 - Lys 231), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human TSLP R, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag with a linear range of 4-31 ng/mL.Biotinylated Human TSLP (R127A, R130A), His,Avitag immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag, can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 1-20 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 50.4 kDa. The protein migrates as 62-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gly 25 - Lys 231
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CRLF2 cytokine receptor-like factor 2 [ Homo sapiens ]
• Official Symbol: CRLF2
• Synonyms: CRLF2; cytokine receptor-like factor 2; CRL2; TSLPR; IL-XR; TSLP receptor; P2RY8/CRLF2 fusion; cytokine receptor-like 2; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin receptor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor; CRLF2Y
• Gene ID: 64109
• mRNA Refseq: NM_001012288
• Protein Refseq: NP_001012288
• MIM: 300357
• UniProt ID: Q9HC73