Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin

Cat.No. : CTLA4-371H
Product Overview : Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # NP_005205.2). Predicted N-terminus: Ala 37. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.
Source : HEK293
Species : Human
Tag : MIgG2a Fc
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human B7-1, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Human B7-2, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.5-7.8 ng/mL (Routinely tested).
Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.1-0.8 ng/mL (Routinely tested).
Molecular Mass : 40.6 kDa
Protein length : Ala 37 - Phe 162
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]
Official Symbol : CTLA4
Synonyms : CTLA4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; GRD4; CTLA-4; IDDM12; CELIAC3;
Gene ID : 1493
mRNA Refseq : NM_001037631
Protein Refseq : NP_001032720
UniProt ID : P16410

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