|Product Overview :||Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # NP_005205.2). Predicted N-terminus: Ala 37. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.|
|Tag :||MIgG2a Fc|
|Form :||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Immobilized Human B7-1, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Human B7-2, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.5-7.8 ng/mL (Routinely tested).
Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.1-0.8 ng/mL (Routinely tested).
|Molecular Mass :||40.6 kDa|
|Protein length :||Ala 37 - Phe 162|
|Endotoxin :||Less than 0.1 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Notes :||Please avoid repeated freeze-thaw cycles.|
|Stability :||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name :||CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]|
|Official Symbol :||CTLA4|
|Synonyms :||CTLA4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; GRD4; CTLA-4; IDDM12; CELIAC3;|
|Gene ID :||1493|
|mRNA Refseq :||NM_001037631|
|Protein Refseq :||NP_001032720|
|UniProt ID :||P16410|