| Cat.No. : |
CYP17A1-02H |
| Product Overview : |
Recombinant human CYP17A1 and human CYP-reductase without tag was coexpressed in Escherichia coli.
Amount: 0.5 nmol cytochrome P450 |
| Description : |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. |
| Source : |
E. coli |
| Species : |
Human |
| Bio-activity : |
Cytochrome c Reductase Activity: 25 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Progesterone 17α-hydroxylase
Linear with time up to: 30 min(Determined at 2 µM substrate, using a P450 concentration of 10 pmol/ml)
Linear with P450 up to: 20 pmol/ml(Determined at 2 µM substrate, using an incubation time of 5 min)
Vmax: 2.5 pmol/min/pmol CYP
Km: 0.19 µM |
| Usage : |
For laboratory (research) purposes only. |
| Storage : |
Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
| Concentration : |
P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10.0 mg/ml
Specific P450 Content: 100 pmol/mg protein |
| Storage Buffer : |
50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.