Active Recombinant Human CYP4F2 Protein

• Cat.No.: CYP4F2-10H
• Product Overview: Recombinant human CYP4F2 and human CYP-reductase without tag was coexpressed in Escherichia coli, supplemented with purified human cytochrome b5.
  Amount: 0.5 nmol cytochrome P450

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away.
• Source: E. coli
• Species: Human
• Bio-activity: Cytochrome c Reductase Activity: 161 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Leukotriene B4 ω-hydroxylase
  Linear with time up to: 30 min(Determined at 20 µM substrate, using a P450 concentration of 50 pmol/ml)
  Linear with P450 up to: 50 pmol/ml(Determined at 40 µM substrate, using an incubation time of 10 min)
  Vmax: 2.7 pmol/min/pmol CYP
  Km: 107 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Cytochrome b5 Concentration: 5.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP4F2 cytochrome P450 family 4 subfamily F member 2 [ Homo sapiens (human) ]
• Official Symbol: CYP4F2
• Synonyms: CYP4F2; cytochrome P450 family 4 subfamily F member 2; CPF2; cytochrome P450 4F2; 20-HETE synthase; 20-hydroxyeicosatetraenoic acid synthase; CYPIVF2; arachidonic acid omega-hydroxylase; cytochrome P450, family 4, subfamily F, polypeptide 2; cytochrome P450, subfamily IVF, polypeptide 2; cytochrome P450-LTB-omega; docosahexaenoic acid omega-hydroxylase; leukotriene-B(4) 20-monooxygenase 1; leukotriene-B(4) omega-hydroxylase 1; phylloquinone omega-hydroxylase CYP4F2; EC 1.14.14.1; EC 1.14.14.78; EC 1.14.14.79; EC 1.14.14.94
• Gene ID: 8529
• mRNA Refseq: NM_001082
• Protein Refseq: NP_001073
• MIM: 604426
• UniProt ID: P78329