Recombinant Human DAXX 293 Cell Lysate

• Cat.No.: DAXX-7071HCL

Specification

• Description: Antigen standard for death-domain associated protein (DAXX), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DAXX death-domain associated protein [ Homo sapiens ]
• Official Symbol: DAXX
• Synonyms: DAXX; death-domain associated protein; death associated protein 6; death domain-associated protein 6; DAP6; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein; EAP1; BING2; MGC126245; MGC126246
• Gene ID: 1616
• mRNA Refseq: NM_001141969
• Protein Refseq: NP_001135441
• MIM: 603186
• UniProt ID: Q9UER7
• Chromosome Location: 6p21.3
• Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; HIV-1 Nef: Negative effector of Fas and TNF-alpha, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; IL-6 Signaling Pathway, organism-specific biosystem
• Function: androgen receptor binding; enzyme binding; heat shock protein binding; p53 binding; protein N-terminus binding; protein binding; protein homodimerization activity; receptor signaling protein activity; transcription coactivator activity; transcription factor binding; ubiquitin protein ligase binding