Recombinant Human CRBN/DDB1 Protein, His-tagged

• Cat.No.: DDB1-928H
• Product Overview: Recombinant Human CRBN/DDB1 Protein(Q96SW2(CRBN)&Q16531(DDB1))(M1-L442(CRBN)&M1-H1140(DDB1)), fused with N-terminal His tag, was expressed in Sf9 Insect Cells.

Specification

• Source: Sf9 Insect Cells
• Species: Human
• Tag: N-His
• Protein length: CRBN: M1-L442; DDB1: M1-H1140
• Form: 20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5
• Bio-activity: The CRBN/DDB1 activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration of lenalidomide, CRBN/DDB1 protein, substrate and beads at 25°C for 60 min, then reading ratio 665/620 with BMG.
• Molecular Mass: CRBN: 52.1 kDa; DDB1: 127.0 kDa
• Purity: > 95% by SDS-PAGE
• Storage: Stable for 12 months upon stored at -80°C from the date of receipt. And avoid repeated freeze-thaws cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TRPM8 transient receptor potential cation channel, subfamily M, member 8 [ Homo sapiens ]
• Official Symbol: TRPM8
• Synonyms: TRPM8; transient receptor potential cation channel, subfamily M, member 8; transient receptor potential cation channel subfamily M member 8; trp-p8; LTrpC-6; transient receptor potential p8; short form of the TRPM8 cationic channel; long transient receptor potential channel 6; transient receptor potential subfamily M member 8; TRPP8; LTRPC6; MGC2849
• Gene ID: 79054
• mRNA Refseq: NM_024080
• Protein Refseq: NP_076985
• MIM: 606678
• UniProt ID: Q7Z2W7