Recombinant Human DDR2 protein, His & T7-tagged

• Cat.No.: DDR2-5685H
• Product Overview: Recombinant Human DDR2 aa. (His578~Pro817 (Accession # Q16832)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.4kDa
• Protein length: His578~Pro817 (Accession # Q16832)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: DDR2 discoidin domain receptor tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: DDR2
• Synonyms: DDR2; discoidin domain receptor tyrosine kinase 2; TKT; MIG20a; NTRKR3; TYRO10; discoidin domain-containing receptor 2; CD167 antigen-like family member B; cell migration-inducing protein 20; discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; receptor protein-tyrosine kinase TKT; tyrosine-protein kinase TYRO10; tyrosylprotein kinase
• Gene ID: 4921
• mRNA Refseq: NM_001014796.1
• Protein Refseq: NP_001014796.1
• UniProt ID: Q16832