Active Recombinant Human DEFB4A protein, His-GST-tagged

Cat.No. : DEFB4A-632H
Product Overview : Active Recombinant Human DEFB4A protein(O15263)(24~64 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.
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Species : Human
Source : E.coli
Tag : GST&His
Protein Length : 24-64 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Defensin Beta 2 (DEFb2) is a 7 kDa secreted protein. It exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria. Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM. The human DEFb2 cDNA encodes a 64 aa protein with a 23 aa signal sequence and a 41 aa mature sequence. Besides, Toll Like Receptor 4 (TLR4) has been identified as an interactor of DEFb2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DEFb2 and recombinant human TLR4. Briefly, DEFb2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to TLR4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DEFb2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of DEFb2 and TLR4 was shown, the EC50 for this effect is 0.11 ug/mL.
Molecular Mass : 34.3kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name DEFB4A defensin, beta 4A [ Homo sapiens ]
Official Symbol DEFB4A
Synonyms DEFB4A; defensin, beta 4A; DEFB2, DEFB4, DEFB102, defensin, beta 2, defensin, beta 4; beta-defensin 4A; DEFB 2; HBD 2; SAP1; defensin, beta 2; defensin, beta 4; skin-antimicrobial peptide 1; BD-2; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102;
Gene ID 1673
mRNA Refseq NM_004942
Protein Refseq NP_004933
MIM 602215
UniProt ID O15263

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