Recombinant Human DIAPH3 protein, His-tagged

• Cat.No.: DIAPH3-1483H
• Product Overview: Recombinant Human DIAPH3 protein(500-850 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 500-850 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: DQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHGNKPYL
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: DIAPH3 diaphanous homolog 3 (Drosophila) [ Homo sapiens ]
• Official Symbol: DIAPH3
• Synonyms: DIAPH3; diaphanous homolog 3 (Drosophila); auditory neuropathy, autosomal dominant 1 , AUNA1, diaphanous (Drosophila, homolog) 3; protein diaphanous homolog 3; AN; DRF3; FLJ34705; NSDAN; diaphanous-related formin 3; diaphanous-related formin-3; auditory neuropathy, autosomal dominant 1; DIA2; AUNA1; diap3; mDia2; DKFZp434C0931; DKFZp686A13178
• Gene ID: 81624
• mRNA Refseq: NM_001042517
• Protein Refseq: NP_001035982
• MIM: 614567
• UniProt ID: Q9NSV4