Recombinant Human DICER1 protein, His-tagged

• Cat.No.: DICER1-1780H
• Product Overview: Recombinant Human DICER1 aa. (Leu6~Ser290) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu6~Ser290
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mMNaHCO3 , 500mMNaCl, pH8.3, containing 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 5.2

Gene Information

• Gene Name: DICER1 dicer 1, ribonuclease III [ Homo sapiens (human) ]
• Official Symbol: DICER1
• Synonyms: DICER1; dicer 1, ribonuclease III; DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE; endoribonuclease Dicer; Dicer1, Dcr-1 homolog; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; helicase MOI; helicase with RNAse motif
• Gene ID: 23405
• mRNA Refseq: NM_001195573.1
• Protein Refseq: NP_001182502.1
• UniProt ID: Q9UPY3