| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
1-187aa |
| Description : |
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. |
| Form : |
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol |
| Bio-activity : |
Specific activity is > 2000 pmol/min/μg, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25 centigrade. |
| Molecular Mass : |
23.6 kDa (207aa) confirmed by MALDI-TOF |
| AASequence : |
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND |
| Purity : |
> 95% by SDS-PAGE |
| Applications : |
SDS-PAGE, Enzyme Activity |
| Notes : |
For research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
| Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
1 mg/mL (determined by Bradford assay) |
| Reference : |
1. Loveridge EJ., et al. (2009) Biochemistry. 48(25):5922-33.
2. Hu Y., et al. (2009) J Cell Biol. 185(1):87-100. |
| Publications : |
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