Recombinant Human DNASE1 Protein, GST-tagged

• Cat.No.: DNASE1-2774H
• Product Overview: Human DNASE1 full-length ORF ( NP_005214.2, 1 a.a. - 282 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 57.8 kDa
• AA Sequence: MRGMKLLGALLALAALLQGAVSLKI AAFNIQTFGETKMSNATLVSYIVQI LSRYDIALVQEVRDSHLTAVGKLLD NLNQDAPDTYHYVVSEPLGRNSYKE RYLFVYRPDQVSAVDSYYYDDGCEP CGNDTFNREPAIVRFFSRFTEVREF AIVPLHAAPGDAVAEIDALYDVYLD VQEKWGLEDVMLMGDFNAGCSYVRP SQWSSIRLWTSPTFQWLIPDSADTT ATPTHCAYDRIVVAGMLLRGAVVPD SALPFNFQAAYGLSDQLAQAISDHY PVEVMLK
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease I [ Homo sapiens ]
• Official Symbol: DNASE1
• Synonyms: DNASE1; deoxyribonuclease I; DNL1; deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I; DRNI; FLJ38093; FLJ44902; DKFZp686H0155
• Gene ID: 1773
• mRNA Refseq: NM_005223
• Protein Refseq: NP_005214
• MIM: 125505
• UniProt ID: P24855