Recombinant Human DNM3A protein, GST-tagged

• Cat.No.: DNMT3A-73H
• Product Overview: Recombinant Human DNMT3A protein, fused with N-terminal GST tag, was expressed in insect cell.

Specification

• Description: DNA (cytosine-5)-methyltransferase 3a (DNMT3a) is required for de novo DNA methylation and methylates at both CpG and non-CpG sites.
• Source: Insect cell
• Species: Human
• Tag: GST
• Form: 50 mM Tris/HCl pH 8.0, 125 mM NaCl, 3 mM DTT, 10% glycerol (v/v).
• Molecular Mass: 127.7 kDa
• Protein length: 2-912
• Purity: >80% by SDS-PAGE
• Storage: Store at -80°C. Thaw quickly and store on ice before use. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1
• Chromosome Location: 2p23
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserv
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; chromatin binding; metal ion binding; methyltransferase activity; protein binding; transferase activity; unmethylated CpG bi

Reviews

11/17/2022

Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

09/07/2022

Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.

08/20/2017

This level of support becomes invaluable, allowing researchers to focus on their scientific objectives while receiving expert guidance alongside.

Q&As

Can DNMT3A protein be used as a biomarker for cancer diagnosis?

DNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.

How does DNMT3A impact normal cellular function?

DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.

Can DNMT3A-targeted therapies have off-target effects?

Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.

Are there diagnostic tests for detecting DNMT3A mutations?

Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.

How does DNMT3A contribute to epigenetic changes in cancer cells?

DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.