Cat.No.:	DOT1L-180H
Product Overview:	Recombinant Human DOT1L(1-435 aa) fused with GST tag at N-terminal was expressed in Insect cells.
Description:	DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones. DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis.
Source:	Insect cells
Species:	Human
Tag :	GST
Form:	50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity:	14 nmol/min/mg
Molecular Mass:	78 kDa
Protein length:	1-435 aa
Purity:	>90%
Applications:	Methyltransferase Assay
Stability:	1 year at -70 centigrade from the date of shipment
Storage:	Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1μg/μl

Gene Name:	DOT1L DOT1-like, histone H3 methyltransferase (S. cerevisiae) [ Homo sapiens ]
Official Symbol:	DOT1L
Synonyms:	DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone H3-K7
Gene ID:	84444
mRNA Refseq:	NM_032482
Protein Refseq:	NP_115871
MIM:	607375
UniProt ID:	Q8TEK3
Chromosome Location:	19p13.3
Pathway:	Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function:	DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transcription factor binding; transferase activity;

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