Recombinant Human DPH3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DPH3-1992H
• Product Overview: DPH3 MS Standard C13 and N15-labeled recombinant protein (NP_996662) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a CSL zinc finger-containing protein that is required for dipthamide biosynthesis. The encoded protein is necessary for the initial step in the modification of a histidine residue in elongation factor-2 to diphthamide. This modified residue is a target for ADP ribosylation by the bacterial toxins diphtheria toxin and Pseudomonas exotoxin A. Alternative splicing results in multiple transcript variants that encode the same isoform.
• Molecular Mass: 9.2 kDa
• AA Sequence: MAVFHDEVEIEDFQYDEDSETYFYPCPCGDNFSITKEDLENGEDVATCPSCSLIIKVIYDKDQFVCGETVPAPSANKELVKCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DPH3 diphthamide biosynthesis 3 [ Homo sapiens (human) ]
• Official Symbol: DPH3
• Synonyms: DESR1; DPH3A; KTI11; ZCSL2; DELGIP; DELGIP1
• Gene ID: 285381
• mRNA Refseq: NM_206831
• Protein Refseq: NP_996662
• MIM: 608959
• UniProt ID: Q96FX2