Recombinant Human EEF1G protein, His-tagged

• Cat.No.: EEF1G-01H
• Product Overview: Recombinant human EEF1G protein (1-437aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 52.5 kDa (460aa)
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSMA AGTLYTYPENWRAFKALIAAQYSGA QVRVLSAPPHFHFGQTNRTPEFLRK FPAGKVPAFEGDDGFCVFESNAIAY YVSNEELRGSTPEAAAQVVQWVSFA DSDIVPPASTWVFPTLGIMHHNKQA TENAKEEVRRILGLLDAYLKTRTFL VGERVTLADITVVCTLLWLYKQVLE PSFRQAFPNTNRWFLTCINQPQFRA VLGEVKLCEKMAQFDAKKFAETQPK KDTPRKEKGSREEKQKPQAERKEEK KAAAPAPEEEMDECEQALAAEPKAK DPFAHLPKSTFVLDEFKRKYSNEDT LSVALPYFWEHFDKDGWSLWYSEYR FPEELTQTFMSCNLITGMFQRLDKL RKNAFASVILFGTNNSSSISGVWVF RGQELAFPLSPDWQVDYESYTWRKL DPGSEETQTLVREYFSWEGAFQHVG KAFNQGKIFK
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 mM DTT

Gene Information

• Gene Name: EEF1G eukaryotic translation elongation factor 1 gamma [ Homo sapiens (human) ]
• Official Symbol: EEF1G
• Synonyms: EEF1G; eukaryotic translation elongation factor 1 gamma; EF1G; GIG35; elongation factor 1-gamma; EF-1-gamma; PRO1608; eEF-1B gamma; pancreatic tumor-related protein; translation elongation factor eEF-1 gamma chain
• Gene ID: 1937
• mRNA Refseq: NM_001404
• Protein Refseq: NP_001395
• MIM: 130593
• UniProt ID: Q53YD7