Active Recombinant Human EGF Protein (Asn 971 - Arg 1023)

• Cat.No.: EGF-333H
• Product Overview: Recombinant Human EGF Protein is expressed from E.coli cells. It contains AA Asn 971 - Arg 1023 (Accession # P01133). This protein carries no "tag". The protein has a calculated MW of 6.3 kDa. The protein migrates as 6.3 kDa under reducing (R) condition (SDS-PAGE).

Specification

• Source: E.coli
• Species: Human
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human EGF Protein at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag with a linear range of 0.156-5 μg/mL (QC tested).
• Molecular Mass: 6.3 kDa
• Protein length: Asn 971 - Arg 1023
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; beta-urogastrone; URG; HOMG4
• Gene ID: 1950
• mRNA Refseq: NM_001178130
• Protein Refseq: NP_001171601
• MIM: 131530
• UniProt ID: P01133