Recombinant Human EGF Protein (Asn 971 - Arg 1023)

Cat.No.: EGF-333H
Product Overview: Recombinant Human EGF Protein is expressed from E.coli cells. It contains AA Asn 971 - Arg 1023 (Accession # P01133). This protein carries no "tag". The protein has a calculated MW of 6.3 kDa. The protein migrates as 6.3 kDa under reducing (R) condition (SDS-PAGE).
Source: E.coli
Species: Human
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Recombinant Human EGF Protein at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His Tag, Avi Tag with a linear range of 0.156-5 μg/mL (QC tested).
Molecular Mass: 6.3 kDa
Protein length: Asn 971 - Arg 1023
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: EGF epidermal growth factor [ Homo sapiens ]
Official Symbol: EGF
Synonyms: EGF; epidermal growth factor; beta-urogastrone; URG; HOMG4;
Gene ID: 1950
mRNA Refseq: NM_001178130
Protein Refseq: NP_001171601
MIM: 131530
UniProt ID: P01133

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