Recombinant Human EGF protein, Mouse IgG Fc-tagged
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|Product Overview :||Recombinant Human EGF (Asn971-Arg1023) protein was fused to mouse IgG2a Fc tag at N-terminus and expressed in human 293 cells (HEK293).|
|Description :||Human epidermal growth factor (EGF) is also known as HOMG4 and URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.|
|Tag :||Mouse IgG Fc|
|Predicted N Terminal :||Asn971|
|Form :||Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 33.1 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Asn971-Arg1023|
|Endotoxin :||Less than 0.1 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of EGF-6282H :||
Human EGF, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of EGF-6282H :||
Immobilized Human EGF R, Fc Tag at 5 μg/mL (100 μL/well)can bind Human EGF, Mouse IgG2a Fc Tag with a linear range of 0.3-10 ng/mL (QC tested).
|Gene Name :||EGF|
|Official Symbol :||EGF|
|Synonyms :||EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4|
|Gene ID :||1950|
|mRNA Refseq :||NM_001178130|
|Protein Refseq :||NP_001171601|
|UniProt ID :||P01133|
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