Active Recombinant Human EGFR protein, His-tagged

• Cat.No.: EGFR-30H
• Product Overview: Active Recombinant Human EGFR protein(P00533-1)(Leu25-Ser645) is expressed from Human Cells with His tag at the C-terminus.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 25-645 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human EGFR, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• Publications:
  • Regulation of EGFR Protein Stability by the HECT-type Ubiquitin Ligase SMURF21,2 (2011)
  • Artificial antibody created by conformational reconstruction of the complementary-determining region on gold nanoparticles (2018)
  • PEGylation of Goldbody: PEG-aided conformational engineering of peptides on gold nanoparticles (2022)

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533