Active Recombinant Human EGFR, His-tagged

• Cat.No.: EGFR-30H
• Product Overview: Recombinant Human EGFR extracellular domain (Met 1-Ser 645) (NP_005219), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His, the EC50 of Human EGFR His is 100-500 ng/mL.
  2.Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 µM as determined in an SPR assay (Biacore T200).
  3.Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His & AVI Tag with an affinity constant of 5.81nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested).
• Molecular Mass: The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
• Protein Length: Met 1-Ser 645
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Artificial antibody created by conformational reconstruction of the complementary-determining region on gold nanoparticles (2018)
  PEGylation of Goldbody: PEG-aided conformational engineering of peptides on gold nanoparticles (2022)
  Regulation of EGFR Protein Stability by the HECT-type Ubiquitin Ligase SMURF21,2 (2011)

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533