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Recombinant Human EGFR Protein, Fc-tagged, Alexa Fluor 488 conjugated
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February 01, 2023 | Add to Quote |
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Cat.No. : | EGFR-024HAF488 Optional Service: Optional requirements on this protein |
Product Overview : | Alexa Fluor 488 conjugated recombinant human EGFR Fc Chimera(rhEGFR-Fc) Met1-Lys642 (Accession # NP_005219) was expressed in human 293 cells (HEK293). |
Description : | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] |
Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Molecular Mass : | rhEGFR-Fc is a homodimeric protein and is fused with the Fc region of human IgG1 at the C-terminus. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. In DTT-reduced SDS-PAGE, protein migrates as 130-140 kDa. |
Protein length : | 860 |
N-terminal Sequence Analysis : | Leu 25 |
Endotoxin : | < 0.01 ng endotoxin/1 μg rhEGFR-Fc. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 488 via amines Excitation Wavelength: 488 nm Emission Wavelength: 515-545 nm |
Storage : | Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhEGFR-Fc should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles. |
Storage Buffer : | Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
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◆ Lysates
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