Active Recombinant Human EGFR Protein, His-Avi-tagged, Biotinylated
| Cat.No. : | EGFR-288H |
| Product Overview : | Biotinylated Human EGFRvIII is expressed from HEK293 cells. It contains AA Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly) (Accession # NP_005219.2). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. |
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| Source : | HEK293 |
| Species : | Human |
| Tag : | His-Avi |
| Predicted N Terminal : | Leu 25 |
| Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : | Immobilized CetuxiMabat 2 ug/mL (100 uL/well) can bind Biotinylated Human EGFRvIII with a linear range of 0.4-25 ug/mL. |
| Molecular Mass : | The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing condition, and 60-70 kDa under non-reducing condition (SDS-PAGE). |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : | >95% as determined by SDS-PAGE. |
| Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
| Official Symbol : | EGFR |
| Synonyms : | EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61; |
| Gene ID : | 1956 |
| mRNA Refseq : | NM_005228 |
| Protein Refseq : | NP_005219 |
| MIM : | 131550 |
| UniProt ID : | P00533 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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