Active Recombinant Human EGFR protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: EGFR-288H
• Product Overview: Recombinant Biotinylated Human EGFR/HER1 Protein(P00533-1)(Leu25-Ser645) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 25-645 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human EGFR, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533