Recombinant Human EGFR protein, His-tagged, Site-Specific AF 488-Labeled

• Cat.No.: EGFR-7532H
• Product Overview: Recombinant Human EGFR protein(NP_005219.2) (Met1-Ser645) was expressed in HEK293 with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max. = 495 nm, Emission Max. = 519 nm).

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ser645
• Form: This product is Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Immobilized Recombinant Human EGF / Epidermal Growth Factor Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human EGFR Protein, the EC50 is 100-300 ng/mL.
  2. Flow cytometric analysis of anti-EGFR CAR expression. 293 cells were lentivirally transduced with anti-EGFR CAR. Flow cytometric analysis was performed with a negative control protein and AF 488-conjugated human EGFR protein (Site-Specific Conjugation, ECD, His Tag, respectively. Non-transduced 293 cells were used as a control (left).
  3. Binding activity of AF 488-conjugated EGFR protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 488-conjugated human EGFR protein and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
• Molecular Mass: The recombinant Human EGFR consists of AA Met1-Ser645 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 72 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Twelve months from date of receipt at -20°C to -70°C in lyophilized form and 3 months at -70°C under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533

Reviews

09/05/2022

The powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.

09/26/2019

Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.

03/23/2019

The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.

Q&As

What biological processes of human embryonic development and tissue regeneration is EGFR associated with?

It is associated with biological processes in human embryonic development and tissue regeneration.

Why does EGFR-inhibiting drug therapy have adverse reactions?

EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.

What is the role of EGFR in tumor treatment?

The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.

What are the general methods used for EGFR gene mutation testing?

EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.

What other drugs can EGFR inhibitors be combined with to increase efficacy?

The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.

How do EGFR mutations affect the efficacy of targeted therapies?

EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.