Recombinant Human EGFR protein, His-tagged, Site-Specific AF 488-Labeled

• Cat.No.: EGFR-7532H
• Product Overview: Recombinant Human EGFR protein(NP_005219.2) (Met1-Ser645) was expressed in HEK293 with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max. = 495 nm, Emission Max. = 519 nm).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser645
• Tag: C-His
• Form: This product is Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Immobilized Recombinant Human EGF / Epidermal Growth Factor Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human EGFR Protein, the EC50 is 100-300 ng/mL.
  2. Flow cytometric analysis of anti-EGFR CAR expression. 293 cells were lentivirally transduced with anti-EGFR CAR. Flow cytometric analysis was performed with a negative control protein and AF 488-conjugated human EGFR protein (Site-Specific Conjugation, ECD, His Tag, respectively. Non-transduced 293 cells were used as a control (left).
  3. Binding activity of AF 488-conjugated EGFR protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 488-conjugated human EGFR protein and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
• Molecular Mass: The recombinant Human EGFR consists of AA Met1-Ser645 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 72 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Twelve months from date of receipt at -20°C to -70°C in lyophilized form and 3 months at -70°C under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533