Recombinant Human EIF2A Protein, GST-tagged

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Recombinant Human EIF2A Protein, GST-tagged

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Cat.No. : EIF2A-3145H
Product Overview : Human EIF2A full-length ORF ( AAH11885.1, 1 a.a. - 585 a.a.) recombinant protein with GST-tag at N-terminal.
Description : This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 91.4 kDa
AA Sequence : MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRESGKNCK VCIFSKDGTL FAWGNGEKVN IISVTNKGLL HSFDLLKAVC LEFSPKNTVL ATWQPYTTSK DGTAGIPNLQ LYDVKTGTCL KSFIQKKMQN WCPSWSEDET LCARNVNNEV HFFENNNFNT IANKLHLQKI NDFVLSPGPQ PYKVAVYVPG SKGAPSFVRL YQYPNFAGPH AALANKSFFK ADKVTMLWNK KATAVLVIAS TDVDKTGASY YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT IFNLKCDPVF DFGTGPRNAA YYSPHGHILV LAGFGNLRGQ MEVWDVKNYK LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSILHKY DVPSNAELWQ VSWQPFLDGI FPAKTITYQA VPSEVPNEEP KVATAYRPPA LRNKPITNSK LHEEEPPQNM KPQSGNDKPL SKTALKNQRK HEAKKAAKQE ARSDKSPDLA PTPAPQSTPR NTVSQSISGD PEIDKKIKNL KKKLKAIEQL KEQAATGKQL EKNQLEKIQK ETALLQELED LKLGI
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : EIF2A eukaryotic translation initiation factor 2A, 65kDa [ Homo sapiens ]
Official Symbol : EIF2A
Synonyms : EIF2A; eukaryotic translation initiation factor 2A, 65kDa; eukaryotic translation initiation factor 2A; EIF 2A; 65 kDa eukaryotic translation initiation factor 2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089;
Gene ID : 83939
mRNA Refseq : NM_032025
Protein Refseq : NP_114414
MIM : 609234
UniProt ID : Q9BY44

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