| Cat.No. : |
EIF2AK2-3148H |
| Product Overview : |
Human EIF2AK2 (NP_002750.1, 252 a.a. - 551 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. |
| Description : |
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Source : |
Insect cells |
| Species : |
Human |
| Tag : |
GST |
| Form : |
Liquid |
| Bio-activity : |
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).
Substrate: SRPKtide.
ATP: 100 μM. |
| Molecular Mass : |
62 kDa |
| Purity : |
61 % by SDS-PAGE/CBB staining |
| Applications : |
Functional Study
SDS-PAGE |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol) |
| Quality Control Testing : |
Loading 1 μg protein in SDS-PAGE
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| Result of activity analysis : |
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