Recombinant Human EIF2AK4 Protein, GST-tagged

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Recombinant Human EIF2AK4 Protein, GST-tagged

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Cat.No. : EIF2AK4-3152H
Product Overview : Human EIF2AK4 (NP_Q9P2K8.2, 1 a.a. - 1649 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]
Source : Insect cells
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein and tRNA, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG.
Substrate: EIF2S1.
ATP: 100 μM.
Molecular Mass : 215 kDa
Purity : 90 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : EIF2AK4 eukaryotic translation initiation factor 2 alpha kinase 4 [ Homo sapiens ]
Official Symbol : EIF2AK4
Synonyms : EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; KIAA1338; GCN2-like protein; GCN2 eIF2alpha kinase;
Gene ID : 440275
mRNA Refseq : NM_001013703
Protein Refseq : NP_001013725
MIM : 609280
UniProt ID : Q9P2K8

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