Recombinant Human EOGT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EOGT-3069H
• Product Overview: C3orf64 MS Standard C13 and N15-labeled recombinant protein (NP_775925) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 52.3 kDa
• AA Sequence: MLMLFVFGVLLHEVSLSGQNEAPPN THSIPGEPLYNYASIRLPEEHIPFF LHNNRHIATVCRKDSLCPYKKHLEK LKYCWGYEKSCKPEFRFGYPVCSYV DMGWTDTLESAEDIFWKQADFGYAR ERLEEMHVLCQPKETSDSSLVCSRY LQYCRATNLYLDLRNIKRNHDRFKE DFFQSGEIGGHCKLDIRTLTSEGQR KSPLQSWFAELQSYTQLNFRPIEDA KCDIVIEKPTYFMKLDAGVNMYHHF CDFINLYITQHVNNSFSTDVYIVMW DTDGKIRVTILARSTEYRKILNQNE LVNALKTVSTFEVQIVDYKYRELGF LDQLRITHNTDIFIGMHGAGLTHLL FLPDWAAVFELYNCEDERCYLDLAR LRGVHYITWRRQNKVFPQDKGHHPT LGEHPKFTNYSFDVEEFMYLVLQAA DHVLQHPKWPFKKKHDELTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EOGT EGF domain specific O-linked N-acetylglucosamine transferase [ Homo sapiens (human) ]
• Official Symbol: EOGT
• Synonyms: EOGT; EGF domain specific O-linked N-acetylglucosamine transferase; AOS4; AER61; EOGT1; C3orf64; EGF domain-specific O-linked N-acetylglucosamine transferase; AER61 glycosyltransferase; EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase; EGF-O-GlcNAc transferase; extracellular O-linked N-acetylglucosamine transferase; EC 2.4.1.255
• Gene ID: 285203
• mRNA Refseq: NM_173654
• Protein Refseq: NP_775925
• MIM: 614789
• UniProt ID: Q5NDL2