Recombinant Human EP300 protein, Flag-tagged

• Cat.No.: EP300-28677TH
• Product Overview: Recombinant Human EP300 fused with Flag tag was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Description: Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with a variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as tumor suppressors. The ability of p300 to acetylate many transcription factors, including p53, E2F, TFIIE, and TFIIF etc. demonstrates a novel mechanism of targeted p300 regulation of gene expression.
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Molecular Mass: 265.2 kDa
• Purity: SDS-PAGE 90%
• Unit Definition: 1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• Applications: Recombinant p300 can be used for: 1) protein-protein interaction assay; 2) in vitro transcription assay; 3) in vitro acetylation assay; and 4) cell growth assay.
• Storage: Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.
• Publications:
  EP300 regulates the expression of human survivin gene in esophageal squamous cell carcinoma. (2016)

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; histone acetyltransferase p300; KAT3B; p300; p300 HAT; E1A-binding protein, 300kD; E1A-associated protein p300; RSTS2
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q09472
• Chromosome Location: 22q13.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: DNA binding; NF-kappaB binding; RNA polymerase II activating transcription factor binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; acetyltransferase activity; activating transcription factor binding; androgen receptor binding; antigen binding; beta-catenin binding; chromatin DNA binding; chromatin binding; core promoter binding; glucocorticoid receptor binding; histone acetyltransferase activity; lysine N-acetyltransferase activity; metal ion binding; mitogen-activated protein kinase binding; peroxisome proliferator activated receptor binding; protein binding; protein complex binding; transcription coactivator activity; contributes_to transcription coactivator activity; transcription factor binding; transferase activity, transferring acyl groups; zinc ion binding