"EPHA3" Related Products

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Recombinant Human EPHA3 Protein, GST-tagged

Cat.No. : EPHA3-3381H
Product Overview : Human EPHA3 (NP_005224.2, 1 a.a. - 464; 1 a.a. - 216 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Description : This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]
Source : Insect cells
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate: Blk/Lyntide.
ATP: 100 μM.
Molecular Mass : 73 kDa
Purity : 88 % by SDS-PAGE/CBB staining.
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : EPHA3 EPH receptor A3 [ Homo sapiens ]
Official Symbol : EPHA3
Synonyms : EPHA3; EPH receptor A3; EphA3 , ETK, ETK1, TYRO4; ephrin type-A receptor 3; HEK; HEK4; EPH-like kinase 4; human embryo kinase 1; eph-like tyrosine kinase 1; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1; EK4; ETK; ETK1; TYRO4;
Gene ID : 2042
mRNA Refseq : NM_005233
Protein Refseq : NP_005224
MIM : 179611
UniProt ID : P29320

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