Active Recombinant Human EPHB2 protein

• Cat.No.: EPHB2-28579TH
• Product Overview: Active Recombinant Human EPHB2 protein(NP_059145.2) (Met 1-Leu 543) was expressed in HEK293 with six amino acids (ENLYFQ ) at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 1-543 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0.
• Bio-activity: Immobilized human EPHB2 at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch, The EC50 of human EFNB2-Fch is 18.2-42.7 ng/ml.
• Molecular Mass: The recombinant human EPHB2 consists of 532 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 66 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB2 EPH receptor B2 [ Homo sapiens ]
• Official Symbol: EPHB2
• Synonyms: EPHB2; EPH receptor B2; DRT, EphB2 , EPHT3, ERK; ephrin type-B receptor 2; Hek5; Tyro5; EPH-like kinase 5; eph tyrosine kinase 3; elk-related tyrosine kinase; protein-tyrosine kinase HEK5; tyrosine-protein kinase TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase receptor EPH-3; developmentally-regulated Eph-related tyrosine kinase; DRT; EK5; ERK; CAPB; PCBC; EPHT3; MGC87492
• Gene ID: 2048
• mRNA Refseq: NM_004442
• Protein Refseq: NP_004433
• MIM: 600997
• UniProt ID: P29323