Recombinant Human Ephrin-A5

• Cat.No.: EFNA5-1611H

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Description: Ephrin-A5, also known as EFNA5, is a member of the ephrin gene family that prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin-A5 is required for the proper guidance and mapping of retinal axons in the mammalian midbrain.
• Molecular Weight: The predicted molecular weight of Recombinant Human Ephrin-A5 is 48.6 kDa. However, the actual molecular weight as observed by migration on SDS Page is 50-55 kDa.
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: EFNA5 ephrin-A5 [ Homo sapiens ]
• Synonyms: EFNA5; ephrin-A5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7 ; eph-related receptor tyrosine kinase ligand 7; LERK-7; AL-1; LERK-7; ephrin-A5
• Gene ID: 1946
• mRNA Refseq: NM_001962
• Protein Refseq: NP_001953
• MIM: 601535
• UniProt ID: P52803
• Chromosome Location: 5q21
• Pathway: Axon guidance
• Function: ephrin receptor binding

Citation

Spatial control of membrane receptor function using ligand nanocalipers.

Journal: Nature methods    PubMed ID: 24997862    Data: 2014/9/29

Authors: Alan Shaw, Vanessa Lundin, Ana I Teixeira

Article Snippet:Prior to EphA2 receptor stimulation, the cells were serum starved for 1 h. Ephrin-A5-Fc homodimers (recombinant human ephrin-A5-Fc chimera, R&D Systems, cat. no. 374-EA-200) were preclustered with IgG antibodies (polyclonal AffiniPure Goat Anti-Human IgG, Jackson ImmunoResearch Laboratories Inc., cat no. 109-005-008) at a mass ratio of 1:10 for 15 min at room temperature, which is a commonly used method to cluster ephrin ligands, hereafter referred to as IgG-clustered ephrin-A5.homodimers (recombinant human ephrin-A5-Fc chimera, R&D Systems, cat. no. 374-EA-200) were preclustered with IgG antibodies (polyclonal AffiniPure Goat Anti-Human IgG, Jackson ImmunoResearch Laboratories Inc., cat no. 109-005-008) at a mass ratio of 1:10 for 15 min at room temperature, which is a commonly used method to cluster ephrin ligands, hereafter referred to as IgG-clustered ephrin-A5. ... Cells were treated for 15 min at 37 °C with either 5 μg/ml IgG or 0.5 μg/ml of ephrin-A5 monomer (Creative BioMart), ephrin-A5 dimer or IgG-clustered ephrin-A5.. For experiments with nanocalipers, cells were treated with 10 nM of ephrin-A5-Fc, which corresponds to 0.5 μg/ml, conjugated to DNA nanocalipers (NC0, NC100, NC40 and NC-sat) or empty nanocalipers (NCempty) as controls.For experiments with nanocalipers, cells were treated with 10 nM of ephrin-A5-Fc, which corresponds to 0.5 μg/ml, conjugated to DNA nanocalipers (NC0, NC100, NC40 and NC-sat) or empty nanocalipers (NCempty) as controls.