Active Recombinant Human EPOR protein, His-tagged

• Cat.No.: EPOR-3851H
• Product Overview: Active Recombinant Human EPOR protein(Met 1-Pro 250)(NP_000112.1) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-250 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 15-60 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO.
• Molecular Mass: The secreted recombinant human EPOR consists of 237 amino acids and has a predicted molecular mass of 26.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEPOR is approximately 34 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPOR erythropoietin receptor [ Homo sapiens ]
• Official Symbol: EPOR
• Synonyms: EPOR; erythropoietin receptor; EPO-R; MGC138358
• Gene ID: 2057
• mRNA Refseq: NM_000121
• Protein Refseq: NP_000112
• MIM: 133171
• UniProt ID: P19235