Recombinant Human ERBB2 protein, Fc-tagged, APC labeled

• Cat.No.: ERBB2-40HA
• Product Overview: Recombinant Human ERBB2 protein, Fc-tagged was Conjugated with NH2-Reactive APC(Allophycocyanin).

Specification

• Description: NH2-Reactive Allophycocyanin is used for antibody conjugation for immunostaining and cellular proteins for tracing. NH2-Reactive Allophycocyanin has succinimidyl ester groups, and can easily make a covalent bond with amino groups of protein without any activation process.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Liquid in sterile PBS buffer pH 7.4 (may contains tiny amount of reaction buffer and Allophycocyanin).
• Endotoxin: <1EU/ug by LAL method.
• Stability: Sample is stable for at least 12 months when stored at -20 to -80°C from date of manufacture. For longer storage, add equal volume of glycerol to the sample solution and store at -20 to -80°C.
• Storage: Store under sterile conditions at -20 to -80°C immediately after receipt. It is recommended that the protein be aliquoted for optimal storage. Avoid freeze-thaw cycles.
• Related Product: ERBB2-40H
• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NGL, v erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); receptor tyrosine-protein kinase erbB-2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NGL; TKR1; HER-2; MLN 19; HER-2/neu

Gene Information

• Official Symbol: ERBB2
• Synonyms: ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NGL, v erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); receptor tyrosine-protein kinase erbB-2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NGL; TKR1; HER-2; MLN 19; HER-2/neu

Reviews

05/09/2022

Worked as expected, satisfied.

03/16/2022

Reliable in protein assays.

09/27/2020

Quick and reliable shipping.

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.

What is the primary role of ERBB2 in cell signaling?

ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.

What therapeutic agents target ERBB2 in cancer treatment?

Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.

In which types of cancer is ERBB2 commonly overexpressed?

ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.

How does ERBB2 overexpression affect patient prognosis in cancer?

ERBB2 overexpression is often associated with aggressive disease and poor prognosis.

How can ERBB2 levels be assessed in a clinical setting?

ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.

How is ERBB2 involved in cell growth and proliferation?

It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.

Are there known genetic mutations that lead to ERBB2 overexpression?

Amplification of the ERBB2 gene leads to its overexpression in cancer cells.