Active Recombinant Human F11 Protein, His-tagged, Low Endotoxin

• Cat.No.: F11-4100H
• Product Overview: Low Endotoxin Active Recombinant Human F11 Protein(P03951-1)(19-625 aa) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-625 aa
• Form: Lyophilized from 0.22 um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5).
• Bio-activity: 1. Immobilized Human Coagulation factor XI, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. 2. Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug.
• Molecular Mass: The protein has a predicted MW of 69.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Gene Information

• Gene Name: F11 coagulation factor XI [ Homo sapiens ]
• Official Symbol: F11
• Synonyms: F11; coagulation factor XI; FXI; plasma thromboplastin antecedent; PTA; MGC141891
• Gene ID: 2160
• mRNA Refseq: NM_000128
• Protein Refseq: NP_000119
• MIM: 264900
• UniProt ID: P03951