Active Recombinant Human F11 protein(C500S), His-tagged, Low Endotoxin

• Cat.No.: F1125452H
• Product Overview: Recombinant Human F11 protein(P03951-1)(Ile388-Val625 (C500S)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 388-625 aa
• Form: Lyophilized from 0.22 μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Coagulation factor XI (C500S), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28.43 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: F11 coagulation factor XI [ Homo sapiens ]
• Official Symbol: F11
• Synonyms: F11; coagulation factor XI; FXI; plasma thromboplastin antecedent; PTA; MGC141891
• Gene ID: 2160
• mRNA Refseq: NM_000128
• Protein Refseq: NP_000119
• MIM: 264900
• UniProt ID: P03951