Recombinant Human FAP Protein, His-tagged FAP-371H

Recombinant Human FAP Protein, His-tagged

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Recombinant Human FAP Protein, His-tagged

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Cat.No. : FAP-371H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human FAP isoform 1 (Q12884-1) extracellular domain (Leu26-Asp760) was fused with the polyhistidie-tag at the N-terminus. The purified protein was biotinylated in vitro.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4
Bio-activity : Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).
The specific activity is >1200 pmol/min/μg
Molecular Mass : The recombinant human FAP consists of 751 amino acids and predicts a molecular mass of 87.2 kDa. As a result of glycosylation, rh FAP migrates as approximately 90 kDa band in SDS-PAGE under reducing conditions.
Protein Length : Leu26-Asp760
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : FAP fibroblast activation protein, alpha [ Homo sapiens ]
Official Symbol : FAP
Synonyms : FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158;
Gene ID : 2191
mRNA Refseq : NM_004460
Protein Refseq : NP_004451
MIM : 600403
UniProt ID : Q12884
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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