Recombinant Human FAP Protein, His-tagged
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|Product Overview :||Recombinant Human FAP isoform 1 (Q12884-1) extracellular domain (Leu26-Asp760) was fused with the polyhistidie-tag at the N-terminus. The purified protein was biotinylated in vitro.|
|Description :||Seprase, also known as 170 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to the peptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP col centigradealized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP col centigradealized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-ass centigradeiated molecules. In ass centigradeiation with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Full Name fibroblast activation protein, alpha Research Areas
Mori,Y. et al., 2004, Oncology. 67 (5-6):411-9.
Aertgeerts K., et al., 2005, J. Biol. Chem. 280:19441-19444.
Liu T., et al., 2005, J. Proteome Res. 4:2070-2080.
Ghersi G., et al., 2006, Cancer Res. 66:4652-4661.
O'Brien,P. et al., 2008, Bi centigradehim Biophys Acta. 1784 (9):1130-45.
|Predicted N Terminal :||His|
|Form :||Lyophilized from sterile PBS. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.|
|Molecular Mass :||The recombinant human FAP consists of 751 amino acids and predicts a molecular mass of 87.2 kDa.|
|Protein length :||Leu26-Asp760|
|Endotoxin :||< 1.0 EU per μg protein as determined by the LAL method.|
|Purity :||> 85 % as determined by SDS-PAGE|
|Storage :||Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Gene Name :||FAP fibroblast activation protein, alpha [ Homo sapiens ]|
|Official Symbol :||FAP|
|Synonyms :||FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158;|
|Gene ID :||2191|
|mRNA Refseq :||NM_004460|
|Protein Refseq :||NP_004451|
|UniProt ID :||Q12884|
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