Active Recombinant Human FAP Protein, His-tagged

• Cat.No.: FAP-006H
• Product Overview: Recombinant human FAP, fused to His-tag at C-terminus, was expressed in insert cell and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 5,000 pmol/min/μg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Z-GP-AMC per minute at pH 7.5, at 37 centigrade.
• Molecular Mass: 86.1 kDa
• AA Sequence: LRPSRVHNSEENTMRALTLKDILNG TFSYKTFFPNWISGQEYLHQSADNN IVLYNIETGQSYTILSNRTMKSVNA SNYGLSPDRQFVYLESDYSKLWRYS YTATYYIYDLSNGEFVRGNELPRPI QYLCWSPVGSKLAYVYQNNIYLKQR PGDPPFQITFNGRENKIFNGIPDWV YEEEMLATKYALWWSPNGKFLAYAE FNDTDIPVIAYSYYGDEQYPRTINI PYPKAGAKNPVVRIFIIDTTYPAYV GPQEVPVPAMIASSDYYFSWLTWVT DERVCLQWLKRVQNVSVLSICDFRE DWQTWDCPKTQEHIEESRTGWAGGF FVSTPVFSYDAISYYKIFSDKDGYK HIHYIKDTVENAIQITSGKWEAINI FRVTQDSLFYSSNEFEEYPGRRNIY RISIGSYPPSKKCVTCHLRKERCQY YTASFSDYAKYYALVCYGPGIPIST LHDGRTDQEIKILEENKELENALKN IQLPKEEIKKLEVDEITLWYKMILP PQFDRSKKYPLLIQVYGGPCSQSVR SVFAVNWISYLASKEGMVIALVDGR GTAFQGDKLLYAVYRKLGVYEVEDQ ITAVRKFIEMGFIDEKRIAIWGWSY GGYVSSLALASGTGLFKCGIAVAPV SSWEYYASVYTERFMGLPTKDDNLE HYKNSTVMARAEYFRNVDYLLIHGT ADDNVHFQNSAQIAKALVNAQVDFQ AMWYSDQNHGLSGLSTNHLYTHMTH FLKQCFSLSD
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -85 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Gene Information

• Gene Name: FAP fibroblast activation protein alpha [ Homo sapiens (human) ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein alpha; FAPA; SIMP; DPPIV; FAPalpha; prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound; gelatinase; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; surface-expressed protease; EC 3.4.14.5; EC 3.4.21.26
• Gene ID: 2191
• mRNA Refseq: NM_004460
• Protein Refseq: NP_004451.2
• MIM: 600403
• UniProt ID: Q12884