Recombinant Human FAP Protein, His-tagged

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Recombinant Human FAP Protein, His-tagged

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Cat.No. : FAP-006H
Product Overview : Recombinant human FAP, fused to His-tag at C-terminus, was expressed in insert cell and purified by using conventional chromatography techniques.
Description : The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Source : Insect cells
Species : Human
Tag : His
Form : Liquid
Bio-activity : Specific activity is > 5,000 pmol/min/μg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Z-GP-AMC per minute at pH 7.5, at 37 centigrade.
Molecular Mass : 86.1 kDa
AA Sequence : <ADP>LRPSR VHNSEENTMR ALTLKDILNG TFSYKTFFPN WISGQEYLHQ SADNNIVLYN IETGQSYTIL SNRTMKSVNA SNYGLSPDRQ FVYLESDYSK LWRYSYTATY YIYDLSNGEF VRGNELPRPI QYLCWSPVGS KLAYVYQNNI YLKQRPGDPP FQITFNGREN KIFNGIPDWV YEEEMLATKY ALWWSPNGKF LAYAEFNDTD IPVIAYSYYG DEQYPRTINI PYPKAGAKNP VVRIFIIDTT YPAYVGPQEV PVPAMIASSD YYFSWLTWVT DERVCLQWLK RVQNVSVLSI CDFREDWQTW DCPKTQEHIE ESRTGWAGGF FVSTPVFSYD AISYYKIFSD KDGYKHIHYI KDTVENAIQI TSGKWEAINI FRVTQDSLFY SSNEFEEYPG RRNIYRISIG SYPPSKKCVT CHLRKERCQY YTASFSDYAK YYALVCYGPG IPISTLHDGR TDQEIKILEE NKELENALKN IQLPKEEIKK LEVDEITLWY KMILPPQFDR SKKYPLLIQV YGGPCSQSVR SVFAVNWISY LASKEGMVIA LVDGRGTAFQ GDKLLYAVYR KLGVYEVEDQ ITAVRKFIEM GFIDEKRIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV SSWEYYASVY TERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT ADDNVHFQNS AQIAKALVNA QVDFQAMWYS DQNHGLSGLS TNHLYTHMTH FLKQCFSLSD <HHHHHH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Enzyme Activity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -85 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Gene Name : FAP fibroblast activation protein alpha [ Homo sapiens (human) ]
Official Symbol : FAP
Synonyms : FAP; fibroblast activation protein alpha; FAPA; SIMP; DPPIV; FAPalpha; prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound; gelatinase; dipeptidyl peptidase FAP; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; seprase; surface-expressed protease; EC 3.4.14.5; EC 3.4.21.26
Gene ID : 2191
mRNA Refseq : NM_004460
Protein Refseq : NP_004451.2
MIM : 600403
UniProt ID : Q12884

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