Active Recombinant Human FCER1A protein, hFc-tagged

• Cat.No.: FCER1A-5467H
• Product Overview: Active Recombinant Human FCER1A protein(P12319-1)(Val26-Gln205) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 26-205 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IgE, His Tag with the EC50 of 1.2ng/ml determined by ELISA..
• Molecular Mass: The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
• Official Symbol: FCER1A
• Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI
• Gene ID: 2205
• mRNA Refseq: NM_002001
• Protein Refseq: NP_001992
• MIM: 147140
• UniProt ID: P12319