Recombinant Human FCER1A protein, hFc-tagged

• Cat.No.: FCER1A-5467H
• Product Overview: Recombinant Human FCER1A protein(P12319-1)(Val26-Gln205), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Val26-Gln205
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
• Official Symbol: FCER1A
• Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI
• Gene ID: 2205
• mRNA Refseq: NM_002001
• Protein Refseq: NP_001992
• MIM: 147140
• UniProt ID: P12319