Recombinant Human FCGR1A, His-tagged, Biotinylated


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Recombinant Human FCGR1A, His-tagged, Biotinylated

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Cat.No. : FCGR1A-180H
Product Overview : Biotinylated Human Fc gamma RI / CD64 Protein (FCA-H82E2) is expressed from human HEK293 cells. It contains AA Gln 16 - Pro 288 (Accession # AAH32634).
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/mL.
Molecular Mass : This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 34.3 kDa. The predicted N-terminus is Gln 16. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
Official Symbol : FCGR1A
Synonyms : FCGR1A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; CD64; CD64A; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; FCRI; IGFR1; FLJ18345;
Gene ID : 2209
mRNA Refseq : NM_000566
Protein Refseq : NP_000557
MIM : 146760
UniProt ID : P12314
Chromosome Location : 1q21.2-q21.3
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; Cross-presentation of soluble exogenous antigens (endosomes), organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem;
Function : IgG binding; protein binding; receptor activity; receptor signaling protein activity;

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